|
培养基和添加剂
|
RPMI-1640(GIBCO,货号31800022, 添加NaHCO3 1.5g/L, glucose 2.5g/L, Sodium Pyruvate 0.11g/L)，90%；优质胎牛血清，10%。 气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。
|
|
REFERENCE
|
Foley GE et al. Continous culture of human lymphoblasts from peripheral blood of a child with acute leukemia. Cancer 18: 522-529, 1965 Sandstrom PA and Buttke TM. Autocrine production of extracellular catalase prevents apoptosis of the human CEM T-cell line in serum-free medium. Proc. Natl Acad. Sci. USA 90:4708-4712, 1993 PubMed: 93281648 Miranda L et al. Isolation of the human PC6 gene encoding the putative host protease for HIV-1 gpl60 processing in CD4+ T lymphocytes. Proc. Natl. Acad. Sci. USA 93: 7695-7700, 1996 PubMed: 96353880 Adams RA. Formal discussion: the role of transplantation in the experimental investigation of human leukemia and lymphoma. Cancer Res. 27: 2479-2482, 1967 PubMed: 68134846 Uzman BG et ah Morphologic variations in human leukemic lymphoblasts (CCRF-CEM cells) after long-term culture and exposure to chemotherapeutic agents. A study with the electron microscope. Cancer 19:1725-I742, 1966 PubMed: 67(146473 Adams RA et al. Leukemia: serial transplantation of human leukemic lymphoblasts in the newborn Syrian halnster. Cancer Res. 27: 772-783. 1967 PubMed: 68122005 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2~a edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1 q88. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D.,(1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Centers for Disease Control (1993), Biosafety in Microbiological and Biomedical Laboratories Human Health Service Publication No. (CDC) 93-8395. U.S Dept. of Health and Human Services; 3rd Edition U.S. Government Printing Office Washington D.C.
|
